Background: Acacia hydaspica R. Parker, family leguminosae, is a medicinally important plant. Different plant\nparts are used in various ailments in folk medicine. The current study aimed at investigating the in vitro\nantioxidant, anti-hemolytic and anticancer activity of A. hydaspica.\nMethods: Antioxidant potential was assessed using DPPH, ABTS and â�¢OH, scavenging of H2O2, inhibition of\nlipid peroxidation and �²-carotene bleaching inhibition assays. Anti-hemolytic activity was assessed using H2O2\ninduced hemolysis of RBCs. Anticancer potential was assessed using MTT assay. Spectrometric methods and\nHPLC-DAD analysis was performed for phytochemical screening.\nResults: EC50 values based on reduction of DPPH, ABTS and â�¢OH, scavenging of H2O2, inhibition of lipid\nperoxidation and �²-carotene bleaching for AHB, AHE and AHM were generally lower manifesting potential\nantiradical capacities. The fractions also exhibited significant (P <0.001) anti-hemolytic potential. Regarding\nIC50 values for anticancer activity against HCC-38 and MDA-MB-361 cancer cell lines; AHB, AHE and AHM\nexhibited significant (P <0.001) cyto-selection indices. Plant extracts showed no cytotoxicity against normal\nVero cells (IC50 > 250 �¼g/ml). While significant (P <0.001) cytotoxicity was elicited by these extract/fractions\nagainst cancer cell lines. AHE was the most effective and IC50 was found to be 29.9 �± 0.909 �¼g/ml (SI = 9.83)\nand 39.5 �± 0.872 �¼g/ml (SI = 7.44) against MDA-MB-361 and HCC-38 cancer cells respectively. Higher amounts\nof TPC and TFC were exhibited by AHE and AHB as compared to other fractions. Gallic acid, catechin and\nmyricetin were identified in AHE whereas gallic acid and catechin were identified in AHB by HPLC.\nConclusion: The presence of bioactive constituents in AHE and AHB might be responsible for antioxidant,\nanti-hemolytic and anticancer activities.
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